Coding
Part:BBa_K2973019:Design
Designed by: Leandros Tsiotos, Vasiliki Kavvatha Group: iGEM19_Thessaly (2019-09-28)
TEM-optimized beta-lactamase / J23119
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 163
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 783
Design Notes
In order to attain maximum enzymatic activity, we implemented a strong protein expression promoter (BBa_J23119) and an optimized coding sequence for beta-lactamase. For this purpose we cloned the part in pSB1C3 and pSB1K3 high-copy plasmids and in E. coli DH5a cells. For protein expression, we transformed the plasmids into BL21 (DE3) and M15-T7 cells.
Source
https://parts.igem.org/wiki/index.php/Part:BBa_I757010
https://parts.igem.org/Part:BBa_B0034
https://parts.igem.org/wiki/index.php?title=Part:BBa_J23119